Figure 2
From: TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3
Subcellular localization and testicular expression of SRSF5 protein. (a) Localization of SRSF5 and CIRP proteins detected by immunofluorescence microscopy in NIH/3T3 cells cultured in PBS with (+) or without (−) 0.4 M sorbitol at 37 °C for 2 h. Scale bars, 50 μm. (b) Immunohistochemical (IHC) detection of SRSF5 protein in the testis of 6-wk-old C57BL/6 J mouse. Square region was enlarged in the right panel. Sg, spermatogonia; Spc, spermatocytes; rSd, round spermatids; eSd, elongated spermatids. Scale bars, 50 μm. (c) Hematoxylin and eosin (H&E) staining and IHC staining for SRSF5 of human normal testis and testicular germ cell tumors. Representative results are shown. Scale bars, 50 μm. (d) IHC scoring of SRSF5 protein levels in human normal testes and germ cell tumors (data indicate mean ± SEM. See Methods for the numbers of samples). (e) IHC comparison of SRSF5 protein levels between non-seminomas (n = 19) and seminoma components of mixed tumors and pure seminomas (n = 30) (data indicate mean ± SEM). Statistical significance was determined by Student’s t-test. **, P < 0.01.
