Figure 4

Effect of thrombus maturation on thrombolysis. Thrombolysis profiles were measured with the Halo assay method after 30 minutes incubation (Fresh clots) and after 4 hours incubation (Aged clots) with 0.5 U/mL, 0.1 U/mL and 0.01 U/mL plasmin (A), with 400 U/mL, 200 U/mL and 100 U/mL urokinase (C) and with 0.35 nM, 0.7 nM, 1.4 nM, 3.5 nM and 7 nM t-PA (F). Activation times (D and G) and maximal clot lysis rates (B,E and H) were presented for each concentration as mean values ± SD (n = 5, ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).