Figure 3
Cutting off tumor blood supply could not prevent local invasion of tumor. (A) The membrane-wrapped tumor was surrounded by newly formed tumor tissue 4 weeks after implantation. The dashed arrow indicates the implanted tumor. The solid arrow indicates the newly formed tumor tissue. (B) The volume of the original implanted-tumors and the adjacent tumor tissue was quantified using 6 mice. (C) The schematic shows that the newly formed tumor tissue surrounded the implanted primary tumor. (D) Three weeks after tumor implantation, the implanted tumors and the adjacent tissue were isolated and stained with CD31 (red), Ki67 (green), and DAPI (blue). The arrows indicate the cell nuclei crossing through the micro-pores of the membrane. (E,F) Electron microscopy shows the inner-face (tumor side) and outer-face (host side) of the 3.0 μm membrane. Red arrows indicate the cells in the micro-pores. (G) The diagram shows that tumor cells cross the micro-pores via morphological changes.
