Figure 2 | Scientific Reports

Figure 2

From: ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth

Figure 2

Oligomerization of Cry1Ac and Cry1AcMod in BBMV from susceptible and resistant populations of P. xylostella. Cry1Ac or Cry1AcMod activated toxins (1.5 μg) were incubated with BBMV (20 μg) from P. xylostella susceptible Geneva 88 or resistant NO-QAGE, BBMV were recovered by centrifugation and separated by SDS-PAGE after three min heating at 60 °C and revealed in western blot using anti-Cry1Ac antibody as described in Experimental procedures. Lane 1 shows Molecular weight marker; lane 2, BBMV from Geneva 88 without toxin; lane 3, BBMV from NO-QAGE without toxin; lane 4, BBMV from Geneva 88 with Cry1Ac toxin; lane 5, BBMV from NO-QAGE with Cry1Ac toxin; lane 6 is a control of activated Cry1Ac toxin directly loaded in the SDS-PAGE; lane 7, BBMV from Geneva 88 with Cry1AcMod toxin; lane 8, BBMV from NO-QAGE with Cry1AcMod toxin and lane 9 is a control of activated Cry1AcMod toxin directly loaded in the SDS-PAGE.

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