Figure 1 | Scientific Reports

Figure 1

From: Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun

Figure 1

Reduced Lck and CD4 expression in mouse Th2 cells. Naive CD4+ T cells were polarized under Th1 and Th2 conditions for 5 days, rested overnight without APCs, antibodies and cytokines and re-stimulated with anti-CD3 (10 µg/ml) and anti-CD28 (2 µg/ml) antibodies. (A,D) Western blotting analysis of cytoplasmic/cell membrane fraction (A) or total cell lysate (D). Results of a representative experiment of four experiments are shown. (B) Densitometry analysis of Western blot images of resting Th1 and Th2 cells. Average and standard deviation of three (pY394Lck/total Lck ratio) and seven (total Lck/β-Actin and total Fyn/β-Actin ratios) experiments are shown. Mann Whitney U test was used to perform statistical comparisons (only for total Lck/β-Actin and total Fyn/β-Actin ratios). (C) Flow cytometry analysis of Th1 and Th2 cells. Results of a representative experiment (of four experiments) are shown. (E) Lck mRNA expression in Th1 and Th2 cells. Lck V1 and Lck V2–transcripts from the proximal promoter, Lck V3–transcript from the distal promoter. Average and standard deviation of eight independent experiments are shown. Student's t-test was used to perform statistical comparisons.

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