Figure 6

Treatment with r-Irisin recovers bone loss in femurs from hindlimb-suspended mice. Representative microCT-generated section images and calculated cortical and trabecular parameters of femurs obtained from normal loading mice (Rest vehicle-inj), unloaded mice treated with vehicle or recombinant Irisin (first r-Irisin injection after 4 weeks of hindlimb suspension, at the dose of 100 µg kg⁻¹ per week for 28 days) and reloaded mice (Reload veh-inj). Cortical bone parameters included bone mineral density (BMD) and cortical thickness (Ct.Th). Trabecular bone parameters included bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb Sp) and Fractal Dimension. Data are presented as mean ± SEM. n = 6–7 mice per group. All data were normally distributed according to the Shapiro-Wilk normality test and analyzed by one-way ANOVA and Bonferroni’s post hoc analysis. Cohen’s d values were measured for non-significant differences of results and can be found as Supplementary Table S1. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 versus Rest vehicle-injected mice. ^{^} p ≤ 0.05, ^{^^} p ≤ 0.01, ^{^^^} p ≤ 0.001 versus Unload vehicle-injected mice.