Figure 4
From: Requirement for Jagged1-Notch2 signaling in patterning the bones of the mouse and human middle ear
Hearing loss in mice lacking Jag1 in NCCs. (a) A click stimulus test was performed in P18 mice, a stage at which wild-type mice show normal hearing. Compared to Jag1 f/+ (n = 7) and Wnt1-Cre; Jag1 f/+ (n = 7) mice, the threshold at which Wnt1-Cre; Jag1 f/f mice (n = 2) could hear was significantly higher. Each point represents one individually tested ear. **p < 0.01; differences were measured by one-way ANOVA with post-hoc Tukey-Kramer HSD test. Error bars represent standard error of the mean. (b) Auditory brainstem responses were recorded at a range of frequencies in P18 mice. Compared to Jag1 f/+ (n = 8), Jag1 f/f (n = 3), and Wnt1-Cre; Jag1 f/+ (n = 8) mice, Wnt1-Cre; Jag1 f/f mice (n = 4) showed significantly higher thresholds across all frequencies as determined by a one-way ANOVA and subsequent post-hoc Tukey-Kramer HSD test. Hearing thresholds of Wnt1-Cre; Jag1 f/+ mice were similar to Jag1 f/+ and Jag1 f/f control mice at lower frequencies but significantly different from Jag1 f/+ controls at 24 and 32 kHz. Circles represent averages, and lines represent individually tested ears. *p < 0.05, **p < 0.01; error bars represent standard error of the mean.
