Figure 1
From: DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
(A) A ribbon diagram of Dbh complexed with Sso7d and DNA. The location of the four structural domains in Dbh are color coded as follows: green, palm domain; cyan, thumb domain; blue, finger domain; magenta, little finger (LF) domain; red, Sso7d. Sso7d is located at the N-terminus and is covalently linked to the palm domain. Sso7d binds to DNA by placing a triple-stranded beta-sheet across the DNA minor groove. The amino acid sequence of the flexible linker (yellow) is SS(GGGGS)3GM. The DNA substrate is shown in brown. (B) SDS-PAGE of the purified Dbh and the variants. Samples were separated on a 10% Mini-PROTEAN TGXTM precast gel (Bio-Rad) and proteins were visualized after staining with Coomassie Brilliant Blue R-250. Lane 1, protein marker (protein molecular weight (kDa) was showed on the left of the lane 1); Lane2-10, SdbhM76I, SdbhKSKIP241–245RVRKS, SdbhL250V, Sdbh, Dbh, SdbhT37F, SdbhA221S, SdbhI62V and SdbhK337R, respectively.
