Table 1 The mutant residue types and frequencies of the non-conserved amino acids (AA).
From: DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
Position sites | Original | Mutant | Position sites | Original | Mutant |
|---|---|---|---|---|---|
AA | AA/frequency(a) | AA | AA/frequency(a) | ||
12 | F | Y/23, A/1 | 222 | K | R/2 |
33 | Y | F/18 | 241 | K | R/24 |
37 | T | F/16, N/3, P2, R1, S/1, E/1 | 242 | S | V/19, T/1, Q/1, I/2, E/5, K/1 |
39 | T | D/20, T/8, P7, K/2, N/4, R/2, S2 | 243 | K | R/25, I/1, V/2 |
43 | V | I/5 | 244 | I | K/25, V/2, T/1, S/1, H/1 |
57 | A | S/19 | 245 | P | S/18, H/6, N/3 |
58 | G | A/11 | 246 | H | I/19, R/2, Q/1, M/1, F/2, Y/1 |
59 | M | I/14 | 249 | Y | I/17 |
60 | P | A/1, S7 | 250 | L | V/20 |
61 | I | A8, C2, S1, L/3 | 283 | R | A/14, K/1, S/1, T/4, E/4 |
62 | I | V/14, P/6, S/2, Y/1, K/1 | 285 | T | H/14, Y/5, S/3, A/3 |
63 | K | R/3, E/11, Q/2 | 289 | I | V/15 |
66 | Q | E/12, K/13, N/1 | 296 | L | V/26, I/2 |
67 | I | L/3 | 298 | K | R/23 |
76 | M | I/7 | 299 | G | S/5, E/3 |
104 | I | V/1 | 300 | K | R/15, Y/2, I/1 |
187 | I | V/12 | 301 | K | T/23, S/4 |
189 | S | N/10, K/5, D/7, E/2, N/10, T/1 | 302 | F | Y/2 |
190 | V | I/15, M/1, S/4 | 303 | K | P/12, T/6, N/2, G/3 |
215 | E | K/20, V/2, S/1, A/1, I/2 | 322 | L | I/25 |
218 | T | I/20, V/5, L/3 | 325 | R | E/19, K/3, S/2, A/1 |
220 | K | R/2, E/16 | 333 | R | E/1, A/1 |
221 | A | S/9, K/1 | 337 | K | R/28 |
