Figure 5
From: Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research
Performance of different protein assays to measure protein concentration of EV samples. (a) Protein quantification for one EV sample using three colorimetric assays (MicroBCA, BCA, Bradford) was performed opposed to one fluorometric assay (Qubit) (n = 3, *p < 0.003). (b) All fluorometric assays (Qubit, FluoroProfile, NanoOrange) were tested on another EV-sample (n = 3, *p < 0.005). Mean values + SD are indicated. Significant differences were calculated using Student’s t-test with Qubit as reference. Abbreviations: EV: extracellular vesicle. BSA: bovine serum albumin.
