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Figure 1

From: Functional characterization of the meiosis-specific DNA double-strand break inducing factor SPO-11 from C. elegans

Figure 1

Expression and purification of C. elegans SPO-11. (a) The SPO-11 expression vector. (b) Extracts from E. coli cells harboring the SPO-11 expression plasmid grown with or without IPTG were analyzed by 10% SDS-PAGE and stained with Coomassie Blue or Western blot analysis with anti-His antibody. (c) Flow chart of SPO-11 purification. (d) Purified SPO-11 (1.5 μg) was analyzed by SDS-PAGE. (e) Results from MALDI-TOF analysis of purified SPO-11. Identified fragments are underlined.

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