Figure 2 | Scientific Reports

Figure 2

From: Functional characterization of the meiosis-specific DNA double-strand break inducing factor SPO-11 from C. elegans

Figure 2

C. elegans SPO-11 is monomeric in solution. (a) Purified SPO-11 was analyzed in a Superdex 75 10/300 GL column. A plot of the partition coefficient (Kav) versus molar mass with size standards was used to calculate the apparent molecular weight of SPO-11. (b) Analytical ultracentrifugation (AUC) with sedimentation velocity analysis of SPO-11. The experimental data were analyzed by the Sedfit program (version 12.1), which yielded an estimated molecular mass of 48.9 kDa. (c) Sedimentation equilibrium analysis of recombinant SPO-11. The average of molecular mass was estimated to be 50 kDa. The upper part of the figure shows the residual difference between experimental and fitted values by the standard deviation.

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