Figure 5
Lack of effect of magnesium on the DNA binding activity of SPO-11. (a) (i) Sequence alignment of the Toprim domain of SPO-11 from various species including C.elegans (Ce), S. cerevisiae (Sc), S. pombe (Sp), M. musculus (Mm), H. sapiens (Hs), and M. janaschii (Mj). (ii) C. elegans SPO-11 mutants generated in this study. (iii) Purified wild-type (WT), E202A (E/A) and E202A/D255A (ED/AA) SPO-11 proteins (1.5 μg each) were analyzed by SDS-PAGE. (b) The dsDNA binding activity of wild-type SPO-11 and mutants was analyzed with the 100 bp dsDNA in the presence of Mg2+. Symbol: PK, proteinase K.
