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Figure 1

From: Prostaglandin E2 inhibits matrix mineralization by human bone marrow stromal cell-derived osteoblasts via Epac-dependent cAMP signaling

Figure 1

PGE2 inhibits hBMSC-mediated matrix mineralization. (A) Alizarin Red S staining was used to assess the influence of continuous PGE2 treatment on matrix mineralization in hBMSC cultures at 14 and 16 days post-osteogenic induction. *p < 0.01, **p < 0.001 as compared to untreated hBMSCs using ANOVA. (B,C) RT-qPCR was used to determine expression levels of osteogenic differentiation markers RUNX2 and ALP at day 3, 7 and 17 post-osteogenic induction (B), and markers of osteoblast maturation and/or matrix mineralization BGLAP, MGP and SPP1 at day 17 post-osteogenic induction (C). Data were normalized to GUSB and expressed as fold change as compared to non-induced controls at day 0 (value 1) using the comparative C T method. *p < 0.05, **p < 0.01, ***p < 0.001 as compared to untreated hBMSCs using ANOVA. The data represent triplicate determinations and were replicated at least two times. All values are presented as mean ± S.D.

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