Figure 3 | Scientific Reports

Figure 3

From: Prostaglandin E2 inhibits matrix mineralization by human bone marrow stromal cell-derived osteoblasts via Epac-dependent cAMP signaling

Figure 3

PGE2 enhances hBMSC adipogenesis. (A) Oil Red O staining was used to assess the influence of continuous PGE2 treatment on triglyceride accrual in hBMSC cultures at day 17 post-adipogenic induction. *p < 0.001 as compared to untreated hBMSCs using ANOVA. (B) RT-qPCR was used to determine expression levels of adipogenic markers PPARG, FABP4 and CD36 in hBMSCs at day 17 post-adipogenic induction. Data were normalized to GUSB and expressed as fold change as compared to non-induced controls at day 0 (value 1) using the comparative C T method. *p < 0.05, **p < 0.01, ***p < 0.001 as compared to untreated hBMSCs using ANOVA. The data represent triplicate determinations and were replicated at least two times. All values are presented as mean ± S.D.

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