Figure 2
From: Down-regulated GATA-1 up-regulates interferon regulatory factor 3 in lung adenocarcinoma
The interaction of GATA-1 and IRF-3 promoter in vitro and in vivo. (a) EMSA was performed with a DNA probe harboring the GATA-1 element (−82 to −75) on the IRF-3 promoter as described in the methods section. (b) A549 cell lysate was subjected to ChIP assay using anti-GATA-1 (IP: GATA-1) or non-specific IgG (negative control) antibodies. After purification of DNA, the IRF-3 promoter area (−149 to +18) was amplified by semi-quantitative PCR. The full-length gel was presented in Supplementary Figure S1.
