Figure 1
Effects of Myricetin (Myr) and Myricitrin (Myr-gly) on S. aureus growth, initial adhesion and biofilm formation. (a) Chemical structure of the Myr consisting of 3-hydroxyflavone backbone and 6 hydroxyl groups. (b,c) Growth kinetics at different concentrations of Myr (0, 5, 25, 50, 100 and 200 µM) during 24 h against S. aureus Newman and ATCC 6538. (d,e) Initial adherence of S. aureus Newman and ATCC 6538 treated with Myr during 1.5, 3 and 6 h. (f,g) Dose-response curve of biofilm formation tested against S. aureus Newman and ATCC 6538 in the presence of Myr. (h) Chemical structure of the flavonol Myr-gly, corresponding to derivative 3-O-rhamnoside of myricetin. (i,j) Growth kinetics at different concentrations of Myr-gly (0, 5, 25, 50, 100 and 200 µM) during 24 h against S. aureus Newman and ATCC 6538. (k,l) Initial adherence of S. aureus Newman and ATCC 6538 treated with Myr-gly during 1:30, 3 and 6 h. (m,n) Dose-response curve of biofilm formation tested against S. aureus Newman and ATCC 6538 in the presence of Myr-gly. *Represents statistically significant differences (p-value < 0.01) in relation to the control samples. Photos of crystal violet assay: increasing violet color indicates higher biofilm formation.
