Figure 2
CC9 forms i-motif structure at neutral pH. 1D 1H-NMR spectra at different pH. H2O/D2O 90:10, 25 mM phosphate buffer, 100 mM NaCl, T = 5 °C, [oligonucleotide] = 0.7–1 mM (A,B,D–F). CD pH titration of the 11-mer sequences were followed at the ellipticity maximum at pH 4: λ = 265 nm (C0, CC8 and CC9) and λ = 265 nm (C7 and CC0), 25 mM AcONa buffer, 100 mM NaCl, T = 5 °C, [oligonucleotide] = 20 μM (C).
