Table 1 Melting temperature and pHT values of C0, C7, CC0, CC8 and CC9.

From: The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

Name

Sequence

pHT

Tm (°C) pH 5.5

Tm (°C) pH 7

C0

d(TCGTTTCGT)

5.8 ± 0.1

~10

—

C7

d(TCGTTTpsCGT)

5.3 ± 0.1

—

—

CC0

d(TCCGTTTCCGT)

6.5 ± 0.1

29.6 ± 0.1

—

CC8

d(TCCGTTTpsCCGT)

6.1 ± 0.1

28.5 ± 0.1

—

CC9

d(TCCGTTTCpsCGT)

6.7 ± 0.1

37.4 ± 0.1

16.7 ± 0.1

  1. Experimental conditions: [oligonucleotide] = 20 μM, 25 mM of buffer solution (cacodylate (pH 5.5) or phosphate (pH 7)) and 100 mM NaCl.
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