Figure 1 | Scientific Reports

Figure 1

From: The role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding

Figure 1

The Trastuzumab mutants (WT, delT, delE, delTE) used in this study (A) Sequence alignments of the Trastuzumab mutants with CDRs highlighted as follows: Vκ-CDR1 (cyan), Vκ-CDR2 (magenta), and Vκ-CDR3 (orange). The deleted residues T74 and E81 are in blue and green, respectively. The structure of the WT-Fab region is shown in dark gray (heavy chain) and in light gray (κ light chain). Schematics of Her2 binding (at CDRs) and protein L binding (at Vκ-FWR1) are shown. (B) Antibody secretion levels of Trastuzumab mutants as determined by Protein G and L biosensor. Each bar shows the average concentration (μg/ml) of Trastuzumab WT and mutants in cell culture supernatants from three independent experiments using protein G and protein L biosensors, followed by statistical analysis using ANOVA and two tailed T-Test. The ANOVA and T-test results showed that the mutants are significantly different from one another (p < 0.05) within each biosensor data set (i.e. Protein L or G).

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