Figure 2
Binding kinetics profiles of Trastuzumab WT and its mutants from three independent experiments with corresponding average association (Ka), dissociation (Kd) and overall binding kinetic (KD) values with standard deviations. (A) Binding kinetics of decreasing concentration (100 nM to 6.25 nM) of Trastuzumab mutants to protein L biosensor, and (B) binding kinetics of Trastuzumab mutants using anti-human Fc capture biosensor to varying concentrations of Her2 (100 nM to 6.25 nM). Poor binding responses of the delTE mutant against protein L and Her2 produced unreliable binding kinetics values as determined by the Octet software for response value < 0.1.
