Figure 2
13-HODE induced ROS production, ER stress, perturbation of lipid metabolism, and apoptosis in Hepa-1c1c7 cells. Hepa-1c1c7 cells were treated with 13-HODE at 1 μM in the presence or absence of NAC at 10 mM for 3 days. (A) ROS production was analyzed by DCF staining (left panel) and apoptosis was analyzed by TUNEL staining (right panel). (B) Immunoblot of proteins involved in apoptosis. (C) Immunoblot of proteins involved in ER stress. (D) Immunoblot of proteins involved in lipid metabolism. Protein levels were quantitated by NIH image J. All values are denoted as means ± SD (n = 3). Significant differences were indicated by different letters (ANOVA, P < 0.05). DCF: 2′,7′-dichlorodihydrofluorescein diacetate, BF: bright field, TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.
