Figure 5

ALOX15 knockout attenuated alcohol-induced perturbation of lipid metabolism. WT and ALOX15−/− male mice were fed with control (PF) or alcohol (AF) liquid diet for 8 weeks. (A) Representative image of Oil red O staining of liver sections. (B) Liver TG content. (C) Liver FFA concentration. (D) Plasma TG content. (E) Plasma FFA concentration. (F) Immunoblot of proteins involved in lipid metabolism. Protein levels were quantitated by NIH image J. All values are denoted as means ± SD (n = 6 in B-E, n = 3 in F). Significant differences were indicated by different letters (ANOVA, P < 0.05). *P < 0.05, **P < 0.01, ns, not significant (t-test). TG: triglyceride, FFA: free fatty acid.