Figure 4

Ectopic expression of Dpp drives tumorous CySC/CC expansion in the Mer mutant. (A–C) The apical tip of the testis in Mer ^ts1 (A), nos > dpp (B), and Mer ^ts1 ; nos > dpp (C) stained for Tj (Green), Vasa (Blue), Add, and Fas III (Red). Bar, 50 µm. (D) The apical tip of the testis from Mer ^ts1; nos > dpp stained for Zfh-1 (Green), Vasa (Blue), and Fas III (Red). (E) The apical tip of the testis from Mer ^ts1; nos > dpp stained for Tj (Green), Vasa (Blue), and Fas III (Red). Expansion of hub cells are shown by arrows. (F) The apical tip of the testis from Mer ^ts1; nos > dpp stained for Tj (Green), Vasa (Blue), and Fas III (Red). Clusters of Tj⁺ CCs are indicated by white circles. (G and H) Examples of mitotic somatic cells [positive for phosphorylated histone H3 (pH3)] away from the hub (G) and within the expanded hub-like structure (H) in a Mer ^ts1; nos > dpp testis. Hub (*).