Figure 2 | Scientific Reports

Figure 2

From: miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin

Figure 2

Ectopic expression of miR-15a/16 intensifies DNA damage and repair. Comet assay in MCF-7 and MDAMB-231 cells after transfection with miR-15a, miR-16, both miR-15a/16 and anti-miR-15a, anti-miR-16 and both. Etoposide was used to chemically induced DNA damage. Cells were processed for single-cell gel electrophoresis (comet assay) and miR-15a, miR-16 or both miR-15a/16 transfected cells show an increase in DNA damage as compared to cells transfected with scrambled miRNAs and anti-miRs under same damage condition. Bar indicates 150 µm (A,B). Cells with DNA damage that showed prominent tail (Comet) were scored using cometIV software and percentage of tail DNA was plotted (C,D,E,F).

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