Figure 5
From: The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate
Summary of normalized cross correlation amplitudes measured with GUVs with co-reconstituted cyt. bo 3 and ATP synthase. (A) The normalized amplitudes were calculated from samples with cyt. bo 3 labeled with either ATTO 647 N or ATTO 594 (bo 3-bo 3), and cyt. bo 3 labeled with ATTO 647N and ATP-synthase labeled with ATTO 594 (bo 3-ATP s.). (B) Cyt. bo 3 was labeled with Abberior STAR 635. The lipid composition was either 99% DOPC (DOPC, blue) or 84–94% DOPC and 5–15% DOPG (DOPC:G, green), and in addition 1% DPPE functionalized with a biotinyl head group. Measurements were done at pH 7.4 in 10 mM HEPES supplemented with 10 mM NaCl and 100 mM glucose. Error bars represent standard deviation from measurements with five samples.
