Figure 4
Plasma ghrelin and its related hypothalamic mediator factors. (a) Plasma ghrelin; (b) Representative hypothalamic ghrelin protein determined by Western blot; (c) Representative GHSR1α protein determined by Western blot; (d) Hypothalamic NPY relative mRNA level; (e) Hypothalamic AgRP relative mRNA level. A densitometric analysis on separate Western immunoblot analyses summarizing the eight animals per group; AgRP – Agouti-related protein; NPY – Neuropeptide Y; GHSR1α – GH secretagogue receptor 1 alpha. Error bars are s.e.m. Statistical differences were determined by unpaired 2-tailed t test. **p < 0.01, difference between control group (C, blue color) and airway obstruction group (AO, green color). # p < 0.05 difference between AO and obstruction removal (OR).
