Figure 2

CD40 is regulated by miR-424 and miR-503 directly. (a) CD40 mRNA expression in response to overexpression of miR-424 or miR-503 mimics, or inhibition of miR-424 or miR-503 with anti-miRs in HUVECs. (b) CD40 protein expression in response to overexpression of miR-424 or miR-503 mimics or inhibition of miR-424 or miR-503 with anti-miRs in HUVECs. (c) Effect of miR-424 or miR-503 overexpression on luciferase reporter containing either the wildtype of mutagenized 3′UTR of human CD40 gene in HeLa cells. Luciferase activity data for constructs with the wild-type (WT) and mutants (#1 and #2) 3′ UTR constructs are shown. *P < 0.05, **P < 0.01, ***P < 0.001 compared to controls. Error bars, s.e.m. Cropped gel images are shown; uncut gels are included in the Supplementary Information.