Figure 1

IRF5 variants activate IFN reporter. (a) A schematic of different IRF5 isoforms. DBD, DNA binding domain; PEST, region rich in proline (P), glutamic acid (E), serine (S) and threonine (T) residues; IAD, IRF association domain; SRR, Serine-Rich Region. “ ^” represents deleted regions. (b) IRF5 isoforms or mutants were transfected with Lucia reporter and TLR7 into HEK293 cells. After 48 hr, cells were mock treated or stimulated with 10 μg/ml R848 for 16 hr. Then cells were lysed and luciferase activities were examined. All experiments were repeated three times and two-tailed student’s t-test was performed. The protein expression levels were determined by Western Blot.