Figure 7
NKX6.3 is correlated with DNMT1 and Hace1 in gastric mucosae and gastric cancer tissues. (A and B) The relative mRNA expression levels of DNMT1 (A) and Hace1 (B) in noncancerous (Normal) and gastric cancer (Tumor) tissues are illustrated by scatterplot. The median expression level of each group is indicated by horizontal line. (C–E) A positive correlation of NKX6.3 with Hace1 and inverse correlation of DNMT1 expression with NKX6.3 and Hace1 expression. (F and G) Expression levels of DNMT1 (F) and Hace1 (G) in 55 gastric mucosae with H. pylori infection, atrophy and intestinal metaplasia. (H and I) A positive correlation between NKX6.3 and Hace1 expression and inverse correlation between DNMT1 and NKX6.3 expression in non-neoplastic gastric mucosae. (J) Methylation status of Hace1 promoter region examined by MSP. Hypermethylation of Hace1 gene was observed only in the gastric mucosae with H. pylori infection, atrophy, and/or intestinal metaplasia. ***P < 0.001, **P < 0.005, based on the two-way ANOVA, linear progression and Chi-square tests.
