Figure 2

Distribution of Ecrg4 in adult rat heart by real-time PCR. Hearts were harvested from 9 adult rats, specimens of Sinus node, left and right atria (L. and R. At.), and left and right ventricles (L. and R. Vt.) were dissected, which were processed for total RNA isolation and real-time PCR as described in materials and methods. SN and L. At. express the highest level of ECRG4, followed by R. At. and both ventricles. Atria express significantly higher level of ECRG4 than ventricles (n = 9, P < 0.05), and L. At. express higher level of ECRG4 than R. At. (n = 9, P < 0.05). Data were presented as “Mean ± SD”, and the experiments were performed in triplicate and repeated three times.