Figure 5
From: High-throughput rapid-prototyping of low-cost paper-based microfluidics
Glucose assay via calorimetric method. (a) Effect of glucose concentration and volume on color intensity. (b) The quantified results of the effect of glucose concentration on color intensities at constant volumes of 5 µl and 2 µl for chromatography paper and delicate task wipers, respectively. (c) The quantified results of the effect of glucose volume on color intensities at concentrations of 2.5 mM and 20 mM on both chromatography paper and delicate task wipers. (d) Glucose assay tested at different concentrations on plus-shaped patterns on both chromatography paper and delicate task wipers and (e,f) their quantified results. Zone 1 is blank, zone 2 has reagent without KI, and zones 3 and 4 have dried reagent. R1 and R2 are the intensity ratios of zone 1 and zone 2 to the paper (i.e. blank paper color intensity quantified at the outside of plus-shaped patterns using the same image to cancel out the ambient light effects), respectively and R3 is the intensity ratio of zone 3 to zone 4.
