Figure 6
From: RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
Validation of RNA-seq data by qRT-PCR. Total RNA was extracted from the monocytes from independent sets of 10 sarcoidosis patients and 10 healthy controls. Isolated RNAs were and reverse-transcribed using the Reverse Transcription System. The primers targeted (a) ATP6AP1, (b) CYBB, (c) LAMP2, (d) SERPIN1A, (e) RPSA and (f) RPL10A to amplify cDNA using iQSYBR Green Supermix. Relative mRNA levels were calculated by normalizing to β-actin. Box plots represent the normalized expression level of each gene of monocytes of healthy controls and sarcoidosis monocytes. Data were analyzed using the paired, two-tailed Student’s t test and the results were expressed as fold change. *Represents a p value < 0.05 and **signifies a p < 0.001.
