Figure 2
From: Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
Genetic analysis of IFN genes. (A) Clustering patterns of all known chicken type I, II and III IFN genes by neighbour-joining method with bootstrap value at n = 2000. The tree was constructed with only ORF sequences including stop codon, and NCBI accession numbers are shown in each of the analysed sequences. Bootstrap values less than 50% were not shown. (B) Phylogenetic tree of type I, II and III IFNs from different mammals, aves and rodents. The inset highlights the association of chicken IFN-κ with closest avian and non-avian species. NCBI accession numbers used for the analysis are shown in parentheses in each of the sequence in the tree. (C) Alignment of the chicken IFN-κ with IFN-κ protein sequences of bovine (NM_001206423), human (NM_020124) and mouse (NM_199157). Amino acid sequences were aligned using ClustalW algorithm and colour represents similarities in the amino acid properties. Putative cleavage signal is marked with down head arrow and five conserved cysteines are labelled with asterisk. Sequences overlaid with lines represent α-helices (A to E) corresponding to human IFN-κ. (D) Predicted three-dimensional structure of chicken IFN-κ where five α-helices are annotated.
