Figure 4
From: Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
The chIFN-κ mediates an antiviral state in vitro and ex vivo. (A) CEF cells stimulated with increasing concentrations of chIFN-κ, chIFN-β (expressed in heterologous HEK-293) or media only (non-treated Ctrl), were infected with AIV (H9N2/UDL/08) or were left uninfected (non-infected Ctrl). Immunostaining of the NP protein of AIV correlates the virus replication in infected cells and antiviral activities of chIFN-κ and chIFN-β. (B) TOCs were stimulated for antiviral response with 1000 Units of chIFN-κ and chIFN-β before infection with AIV (H9N2/UDL/08). Immunostaining of the NP protein of influenza virus indicate the cellular replication levels of influenza viruses in IFNs treated TOCs compared to non-infected and non-treated controls. (C) Quantitative measurement of AIV ((H9N2/UDL/08) in TOCs supernatants. The data represents 2 or 3 independent experiments.
