Figure 6
From: Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
Spatiotemporal expression dynamics of the transgene in developing embryos, and antiviral activity of chIFN-β and chIFN-κ in ovo. (A) Using experimental layout, depicted in the schematic illustration in the inset, expression of the GFP reporter gene indicated the embryo-wide distribution. After infection of eggs on third day of embryonation (ED), the expression of the GFP was detected as early as 6 days post-infection (PI) and most organs of the embryos were positive for the transgene at day 11 post-infection. Representative images from each time points are shown, and all images are shown at x40 magnification. (B) Experimental layout for the generation of transgenic embryos and analysis of the antiviral effects of IFNs in ovo. (C) Replication of both influenza and ND viruses was markedly compromised in the allantoic fluids collected from eggs expressing RCASBP(A)-mediated chIFN-β and chIFN-κ compared to RCASBP(A)-wt virus. Each dot, circle or triangle represents individual egg and the cross-lines show the mean in each group.
