Figure 7
From: Ancestral protein resurrection and engineering opportunities of the mamba aminergic toxins
Affinity constants of AncTx1 and AncTx3 variants on adrenoceptors. pKi values are reported for AncTx3, AncTx4, ρ-Da1a, AncTx1 and its 2 variants (AncTx1 I38S and W28R-I38S) on the 4 adrenoceptors α1A, α1B, α1D and α2C. The 12-times increase in selectivity for α1A between ρ-Da1a and AncTx1 is shown. AncTx4 and ρ-Da1a are here represented as AncTx3 variants (W28R and W28R-I38S respectively), like I38S and W28R-I38S for AncTx1, in order to compare the effect of these substitutions in AncTx3 and AncTx1.
