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Figure 1

From: Lipid Droplet-Associated Hydrolase Promotes Lipid Droplet Fusion and Enhances ATGL Degradation and Triglyceride Accumulation

Figure 1

LDAH is a cytosolic protein that localizes to LDs upon OA loading. (A) LDAH-GFP (left) or Flag-tagged LDAH (right) were transfected to HeLa cells, and cells were treated with OA (360 μM for 24 h). Anti-Flag antibody (green) was used to detect Flag-LDAH, and LipidTox (red) was used to stain neural lipids. Nuclei were stained with DAPI (blue). Yellow arrows indicate LDAH associated with merged LDs. (B–E) Images of GFP or LDAH-GFP transfected cells loaded with OA were taken by fluorescence microscope and used for morphometric analysis of LDs to determine the effects of LDAH on LD size distribution (B); average size of LDs (C); average number of LDs per cell (D); and total LD area per cell (E). Ten cells per group were used for the measurements. *p < 0.05, **p < 0.005.

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