Figure 2
LDAH increases LD size by promoting fusion in a tubulin-dependent manner. (A) HEK293 cells were transfected with LDAH-GFP, and remained untreated or were incubated with 360 μM of OA for the indicated periods of time. Yellow arrows indicate fused LDs. (B) HEK293 cells were co-transfected with LDAH-GFP (green) and dsRed2-ER (red). Cells remained untreated or were treated with OA (360 μM) for 30 min. (C) LDAH-GFP overexpressing HEK293 cells remained untreated or were pre-treated with brefeldin A (BFA) for 30 min. OA (360 μM) was then added to the culture media for 3 h. LDAH’s localization to LDs was determined by fluorescence microscopy (green). (D) LDAH-GFP expressing HEK293 cells remained untreated or were pre-treated with nocodazole (2 μg/ml) for 30 min. OA (360 μM) was then added for 24 h.
