Figure 4

Comparison of migration track patterns. Representative tracks in the classic under-agarose chemotaxis assay (a) and substrate-bound haptotaxis assay (b). (b) The tracks are first persistent and elongated (in green), followed by deceleration and random walk around the end position (in blue). (c) Time-dependent evolution of the cumulative squared radius of gyration. Time 0 corresponds to the end of the track. Color coding is the same as in (a,b). (d) Fraction of total tracks showing dual behaviour (n = 3 for chemotaxis, n = 8 for haptotaxis assay). *p = 0.0065 (Student’s unpaired t-test). (e) Fraction of still cells in binned regions of gradients (n = 8 experiments). Colors represent individual experiments.