Table 4 Metabolites altered after purified rhodomyrtone treatment of Streptococcus pneumoniae.

From: Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Metabolite

Strain

MW (g/mol)

Retention time (min)

Precursor ion (m/z)

Fold changea

Related pathway or function

Metabolites with decreased abundance

 Thymidine monophosphate

R6, TIGR4

322.057

2.7

321.049

13.49*, 1.71

Nucleic acid biosynthesis

 L-Tryptophan

R6, TIGR4

204.090

4.8

205.097

1.34*, 1.70*

Amino acid biosynthesis

 Uridine 5′-diphosphoglucuronic acid

R6, TIGR4

580.034

1.1

579.028

1.69*, 3.00*

Capsule synthesis

N-Acetyl-L-glutamic acid

R6, TIGR4

189.064

1.8

188.056

2.11*, 1.09

Urea cycle

 UDP-glucose

R6, TIGR4

566.055

1.1

565.047

1.31, 1.27*

Capsule synthesis

 Hypoxanthine

R6, TIGR4

136.039

1.6

137.046

1.02, 2.21*

Nucleic acid biosynthesis

 Deoxyinosine

R6

252.086

3.7

251.077

INFb

Nucleic acid biosynthesis

 Guanosine

R6, TIGR4

283.092

2.2

284.099

1.01, INF

Nucleic acid biosynthesis

 Inosine

R6

268.081

3.3

267.074

1.07

Nucleic acid biosynthesis

 D-(+)-3-Phenyllactic acid

R6, TIGR4

166.063

6.6

165.054

INF, INF

Antimicrobial compound

 L-Aspartic Acid

TIGR4

133.038

1.0

132.030

1.35

Amino acid biosynthesis

 N-Acetyl-DL-methionine

TIGR4

191.062

5.1

190.054

4.77*

Oxidative stress response

 Acetyl-CoA

TIGR4

809.126

5.0

403.556

1.98*

Lipid metabolism, activating intermediate molecule

 Raffinose

R6

504.169

1.2

503.162

2.03*

Raffinose/stachyose/melibiose transport system

 Deoxyadenosine monophosphate

TIGR4

331.068

1.6

332.075

1.04

Nucleic acid biosynthesis

 Palmitic acid

R6, TIGR4

256.240

0.4

257.247

1.09, 1.38

Lipid biosynthesis

 UDP-N-acetyl-D-galactosamine

TIGR4

607.082

1.2

606.074

3.12*

Capsule synthesis

 Pyroglutamic acid

R6

129.043

1.5

128.035

3.77*

Amino acid metabolism

Metabolites with increased abundance

 Guanosine monophosphate

R6, TIGR4

363.058

1.5

362.052

3.38*, 2.32*

Nucleic acid biosynthesis

 L-Tyrosine

R6

181.074

1.8

180.067

1.08

Amino acid biosynthesis

 Uridine monophosphate

R6, TIGR4

580.034

1.1

579.028

4.73*, 1.37*

Nucleic acid biosynthesis

 D-Ribulose 5-phosphate

R6

230.019

0.7

229.012

1.05

Pentose phosphate pathway

 D-Glucose 6-phosphate

R6, TIGR4

260.030

0.7

259.023

1.71, 5.25*

Glycolysis

 Cyclic adenosine diphosphate ribose

R6, TIGR4

541.061

1.4

540.055

2.85*, 1.04

Calcium signaling

 L-Glutamic acid

R6, TIGR4

147.053

0.7

146.046

1.37, 1.44*

Amino acid biosynthesis

 L-Phenylalanine

R6

165.079

3.5

166.086

1.69*

Amino acid biosynthesis

  1. aThe fold change values represent the ratio between control and rhodomyrtone treatment for metabolites with decreased abundance, or the ratio between the rhodomyrtone treatment and the control for metabolites with increased abundance. Statistical significance of the analysis under the Student’s t-test is indicated as *(p < 0.05). bINF means that the metabolite was not detected in the rhodomyrtone-treated samples.
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