Figure 8

IPNV particles co-localize with Dextran-TR. IPNV at a MOI of 10 was adsorbed to serum-starved CHSE-214 cells at 4 °C for 1 h and pulsed with dx-TR for 45 min at 20 °C without (A–D) or with EIPA 20 μM (E–H). After washing, cells were processed for immunofluorescence using oligoclonal anti VP2/Vp3 IPNV antibodies and Alexa fluor®466 secondary antibody and Höechst solution for nuclear staining. Z-stacks were recorded by confocal microscopy and representative maximum Z projections are shown (scale bar = 5 µm). Analyses of co-localisation were conducted with Image J software and resulting co-localised pixel map are shown for each condition (colocalised pixels are shown as white spots) (D and H).