Figure 3 | Scientific Reports

Figure 3

From: Identification of the functional variant driving ORMDL3 and GSDMB expression in human chromosome 17q12-21 in primary biliary cholangitis

Figure 3

Luciferase reporter assay of rs12946510. (A) Outline of the luciferase reporter plasmid constructs used for transfection. The plasmids consisted of PCR fragments of the genomic DNA between GRB7 and IKZF3 containing rs12946510 that were sub-cloned into the pGL4.23 vector. (B and C) The ability of these plasmid constructs to enhance transcription in transfected Jurkat (B) and HepG2 (C) cells was measured by determination of cellular luciferase (luc) activity 24 hours after transfection. The luciferase activities of cells transfected with the PBC susceptibility allele (T-allele) of rs12946510 were reduced compared to those transfected with the major allele (C-allele). Three independent experiments were performed in each assay. The data in the figures represent averages ± standard deviation of triplicate assays in one experiment. *P < 0.001 (Student’s t test).

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