Figure 4 | Scientific Reports

Figure 4

From: Identification of the functional variant driving ORMDL3 and GSDMB expression in human chromosome 17q12-21 in primary biliary cholangitis

Figure 4

Analysis of FOXO1 binding using a super-shift assay. (A and B) Prediction of transcription factor binding by the TRANSFAC professional database. Although the sequence of the FOXO1 binding site contains the major allele of rs12946510 (C allele, A), this sequence was disrupted by the PBC- susceptibility allele (T allele, (B). (C) The binding motif of FOXO1. The nucleotide that is shown inside the rectangle is the position of rs12946510. (D) Incubation of the Jurkat cell nuclear extract with a specific anti-FOXO1 antibody resulted in a super-shift of the shifted band that was observed in the EMSA. Three independent experiments were performed in each assay.

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