Figure 2
Hydrodynamic injection of dual sgRNA cassette accelerated tumor formation in HBV transgenic mice. (A) Cartoon showing hydrodynamic tail vein injection of dual sgRNA cassette to an adult mouse and the experimental design. HDT, Hydrodynamic tail vein injection. G1, group 1; G2, group 2; G3, group 3. (B) Liver tumors detected in the three groups of mice harvested 6 and 8 months’ post injection. Two representative photographs of mouse livers were showed in each group. At 6 months’ post injection, 3 mice in C57-HBV PX458 group, 3 mice in C57-HBV sgp53/Pten group, and 5 mice in C57 sgp53/Pten group were sacrificed. At 8 months’ post injection, the number of the sacrificed mice in the three groups were 3, 4 and 4 respectively. The scale bar is 5 mm. (C) Liver weight relative to the whole body weight of mice 8-month post injection. (D & E) Circulating AFP and ALT level in sera of the mice 8 months’ post injection with PX458 or dual sgRNA cassette. (F) HBsAg expression detection in sera of the C57-HBV mice 8-month post injection. Data represent means ± SEM (n ≥ 3). Statistical significance was determined with Student t two-tailed test. ** indicated P < 0.01.
