Figure 2 | Scientific Reports

Figure 2

From: Platelet-rich plasma respectively reduces and promotes adipogenic and myofibroblastic differentiation of human adipose-derived stromal cells via the TGFβ signalling pathway

Figure 2

PRP profiling. Cytokines released from PRPs were detected using the RayBio C-Series Human Growth factor Antibody kit. Left panel: Images of arrays probed with indicated PRPs. Cytokines are spotted in duplicate and those released from PRP appear as green dots. The positions and names of cytokines that generated a detectable signal are as follows. 1: Positive control; 2: Negative control; 3: EGF; 4: EGFR; 5: GCSF; 6: GM-CSF; 7: IGFBP-1; 8: IGFBP-2; 9: IGFBP-3; 10: IGFBP-6; 11: M-CSFR; 12: PDGF-AA; 13: PDGF-AB; 14: PDGF-BB; 15: SCFR; 16: TGFb1. Cytokines that were present on the array and non detectable are as follows: amphiregulin (AR, AREG), bFGF, b-NGF, FGF-4, FGF-6, FGF-7, GDNF, HB-EGF, HGF, IGFBP-4, IGF-I, IGF-I R, IGF-II, M-CSF, NT-3, NT-4, PDGF R a, PDGF R b, PLGF, SCF, TGF alpha, TGF beta2, TGF beta3,VEGF-A,VEGFR2,VEGFR3,VEGF-D. Right panel: factors/cytokines presenting a detectable fluorescent signal are indicated. Values are the fluorescence means ± SEM (n = 3 individual PRPs.) *p < 0.05; **p < 0.01.

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