Figure 5
From: Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
MCM7 depletion promotes mitotic exit. (A) HepG2 cells were transfected with MCM7 siRNA or negative control siRNA (Control) for 24 h or 72 h. MCM7 protein level was analyzed by Western blot. GAPDH was used as the loading control. (B) HepG2 cells and LO2 cells were transfected with MCM7 siRNA (siRNA) or negative control siRNA (Control) for 12 h, then 0.2 μg/ml nocodazole was added to cell culture for further 12 h and then incubated in fresh medium for 0 h, 1 h, 2 h, 3 h and 4 h. Cell cycle was analyzed by FACS and data are shown as mean ± SD of three independent experiments (C), *P < 0.05. (D) MCM7, p-CDK1 and RAD21 were detected by western-blot. GAPDH was used as the loading control. Data are expressed as optical density (OD) fold difference related to GAPDH from 3 duplicate experiments (E), *P < 0.05.
