Figure 6

MCM7 depletion contributes to aberrant mitosis. (A,B) HepG2 cells were transfected with MCM7 siRNA or negative control siRNA. After being transfected for 12 h, cells treated with 0.2 μg/ml nocodazole for further 12 h and then incubated in fresh medium for 0 h, 0.5 h, 1 h and 2 h. Immunofluorescence staining was performed to detect MCM7 (red) and β-Tubulin (green) in the cells, and nucleuses were visualized by DAPI staining (blue). (C) HepG2 cells were treated with or without 0.2 μg/ml nocodazole for 12 h to block cell in M phase, above immunofluorescence staining was performed and observed under confocal microscopy.