Figure 9
From: Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
Pull-down assay to examine interaction between ClAQP1 and ClGlp1: X. laevis oocytes expressing His-ClAQP1 and myc-ClGlp1 or myc-ClGlp1 alone (control) were used to extract proteins, which were subjected to pull-down assay using Ni2+ NTA agarose matrix (Invitrogen). Presence of myc-ClGlp1 in the eluent was assessed by Western blot. Crude protein extracts were loaded as control.
