Figure 5

Influence of TNF-α-stimulated EC culture supernatants and concentrated pellets with and without incubation of anti-TF antibody on ROTEM parameters. Different fractions of the supernatants from ECs stimulated with TNF-α for 24 h were collected, additionally exposed to a blocking anti-tissue factor antibody (TF-AB) and 50 µl study samples were added to 300 µl fresh whole blood and subjected to ROTEM analysis. EGM-2 = endothelial growth medium-2; PBS = phosphate buffered saline; S0.5 = cell free culture supernatant; P14 = pellet containing larger vesicles; P100 = pellet containing smaller vesicles; S100 = vesicle free supernatant; ns = not significant; *p < 0.05; **p < 0.01; ***p < 0.001; Box-and-whisker plots (min, max, 25th–75th percentile, median). n = 4–6 measurements.